![]() However, present-day preclinical platforms (animals and in vitro models) are not robust enough to provide reliable data for clinical trials. Currently, therapeutic strategies are actively sought and developed. Consequently, at least 40% of such patients die within five years post-amputation. When diabetic foot ulcers are not treated and managed in a timely manner, 15 to 50% of patients tend to undergo partial or complete amputation of the affected limb. Diabetic foot ulceration affects hundreds of millions of individuals globally, especially the elderly, and constitutes a major socio-economic burden. This paper presents key considerations for developing physiologically relevant immunocompetent diabetic foot ulcer models. Thorough investigation of the intricate pathology of skin diseases and the development of effective treatments requires use of highly efficacious models of skin diseases.īioengineering of skin has been significantly explored, ranging from the use of traditional cell culture systems to the most recent organ-on-a-chip technology that permits skin modelling on physiological scales amongst other benefits. In future studies utilizing 3D HSEs, emphasis must be placed on integrating all adnexal structures relevant to the skin disease under investigation. While the field of bioengineered models for study of skin disease has made tremendous progress in the last decade, there are still significant efforts necessary to create truly biomimetic skin disease models. ![]() The development of commercially available 3D bioprinters has allowed researchers to create highly reproducible 3D HSEs with precise integration of multiple adnexal structures. One method of constructing 3D HSEs, 3D bioprinting, has emerged as a versatile and useful tool for generating highly complex HSEs. The addition of adnexal structures to 3D HSEs has allowed researchers to gain more insight into the complex pathology of various hereditary and acquired skin diseases. These 3D HSEs can be highly complex, containing both epidermal and dermal compartments with integrated adnexal structures. ![]() Three-dimensional human skin equivalents (HSEs) have emerged as an advantageous tool for the study of skin disease in vitro. Three-dimensional modeling systems are better able to recapitulate the complex cell–cell and cell–matrix interactions that occur in vivo within skin. ![]() Skin disease research has been shifting from less complex and less relevant 2D (two-dimensional) models to significantly more relevant 3D (three-dimensional) models. Models of skin diseases, such as psoriasis and scleroderma, must accurately recapitulate the complex microenvironment of human skin to provide an efficacious platform for investigation of skin diseases. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |